Asked by India
The total phosphate concentration in a blood sample is determined by spectrophotometry to be 3.0×10−3 M. The pH of the blood sample is 7.45, What are the concentrations of H2PO4− and HPO42- in the blood? Pka for the phosphate system is 7.198
Answers
Answered by
DrBob222
You have two equations.
pH = pKa + log(base)/(acid)
Substitute and solve fr base/acid. Calling base = b and acid = a, you will get a ratio of
b/a = ?, This is equation 1.
equation 2 is
a + b = 3.0E-3
Solve the two equations simultaneously for a and b = (acid) and (base)
note:(acid) is H2PO4^-
(base) is HPO4^2-
pH = pKa + log(base)/(acid)
Substitute and solve fr base/acid. Calling base = b and acid = a, you will get a ratio of
b/a = ?, This is equation 1.
equation 2 is
a + b = 3.0E-3
Solve the two equations simultaneously for a and b = (acid) and (base)
note:(acid) is H2PO4^-
(base) is HPO4^2-
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