You want to know if a new species of bacteria we identified (E. glados) has resistance to the antibiotic valinomycin. You make “plates” of LB-agar to culture bacteria on. Some of these are regular LB-agar plates that any bacteria will grow on and others have valinomycin in them, to prevent growth of bacteria without resistance to this antibiotic. You plate E. glados on the valinomycin-containing LB-agar plates and see that no colonies grow. In this experiment to test if E. glados is valinomycin-resistant, what is the simplest positive control you can perform?
3 answers
Would it be simply if E. glados grows on media without antibioitics in it?
Or would it be growth of E.glados on media with another form of antibiotic resistance on it? I know that it is simplest, but I'm not sure if I'm missing the purpose of a positive control
What is this?