You wish to make 3 reactions (1 ml each) with the specified amounts of protein. The remainder of each reaction consists entirely of buffer. The source of your protein is a stock solution that has a concentration of 0.5 mg/ml. What is the volume of stock solution required for each reaction (column 3). What is the volume of buffer required (column 4)? Show your work. Be sure to pay attention to units.

Question

#Reaction Amount of protein (ug) V of Protein solution(ul)required V of Buffer (ul)required Final V (ml)
1 2 1
2 5 1
3 10 1