You want to create a 1:20 dilution of a bacterial culture. You want a total of 40 ml of diluted bacterial culture, how much culture would you add to how much media?

add 1 ml culture to 20 ml media
add 2 ml culture to 40 ml media
add 2 ml culture to 38 ml media
add 2 ml culture to 42 ml media

1 answer

The correct option would be to add 2 ml of culture to 38 ml of media in order to create a 1:20 dilution of a bacterial culture with a total volume of 40 ml.