To find the protein concentration of the flowthrough fraction, we can use the equation y = 12.235x, where y is the absorbance reading and x is the protein concentration in mg/ml.
Given that the absorbance reading is 0.52 and the protein concentration is 0.03 mo, we can substitute these values into the equation to solve for x.
0.52 = 12.235 * 0.03x
Dividing both sides of the equation by 12.235:
0.52 / 12.235 = 0.03x
0.0425 = 0.03x
Dividing both sides of the equation by 0.03:
0.0425 / 0.03 = x
x ≈ 1.4167
Therefore, the protein concentration of the flowthrough fraction is approximately 1.4167 mg/ml.
You take 0.03 mo of the flowthrough fraction, measure the absorbable using the same conditions as in the standard curve above, and get a reading of 0.52. What is the protein concentration of the flowthrough fraction (mg/ml)? y=12.235x
1 answer