You are carryingout TLC on normal phase plates. Compound A has an Rf of 0.4 and compound B of 0.6. What would happen to the Rf values if you increase the polarity of the mobile phase?
For the compounds in question 1, you change to running them on reverse phase plates. What would you expect to happen.
A compound has a retention time of 3 minutes on GLC at a column tep. of 120 C. What would happen if you increased the column temp. to 160 C.