Question

Write the complementary sequence for the following DNA sequence, in order from 3' to 5':
5′−CGATATTGAGCTAAGCTT−3′


Use all capital letters to write the DNA sequence.
View Available Hint(s)for Part B
3′−

−5′

Answer 1

To find the complementary DNA sequence, you need to pair each base with its complementary base. The base pairing rules are as follows:

- Cytosine (C) pairs with Guanine (G)
- Guanine (G) pairs with Cytosine (C)
- Adenine (A) pairs with Thymine (T)
- Thymine (T) pairs with Adenine (A)

Given the DNA sequence:
5′−CGATATTGAGCTAAGCTT−3′

The complementary bases will be:
- C → G
- G → C
- A → T
- T → A

Now, let's write out the complementary sequence from 3' to 5':

1. C → G
2. G → C
3. A → T
4. T → A
5. A → T
6. T → A
7. G → C
8. A → T
9. G → C
10. C → G
11. T → A
12. A → T
13. A → T
14. G → C
15. C → G
16. T → A

Putting it all together in the 3' to 5' direction, we get:

3′−GCTATAACTCGATTCGAA−5′