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Write the complementary sequence for the following DNA sequence, in order from 3' to 5':

5′−CGATATTGAGCTAAGCTT−3′


Use all capital letters to write the DNA sequence.
View Available Hint(s)for Part B
3′−

−5′

1 answer

To find the complementary DNA sequence, you need to pair each base with its complementary base. The base pairing rules are as follows:

  • Cytosine (C) pairs with Guanine (G)
  • Guanine (G) pairs with Cytosine (C)
  • Adenine (A) pairs with Thymine (T)
  • Thymine (T) pairs with Adenine (A)

Given the DNA sequence: 5′−CGATATTGAGCTAAGCTT−3′

The complementary bases will be:

  • C → G
  • G → C
  • A → T
  • T → A

Now, let's write out the complementary sequence from 3' to 5':

  1. C → G
  2. G → C
  3. A → T
  4. T → A
  5. A → T
  6. T → A
  7. G → C
  8. A → T
  9. G → C
  10. C → G
  11. T → A
  12. A → T
  13. A → T
  14. G → C
  15. C → G
  16. T → A

Putting it all together in the 3' to 5' direction, we get:

3′−GCTATAACTCGATTCGAA−5′