Write a summary on the aim of the journal article 'Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins' written by Ana Cristina Puhl, Cecilia Giacomini, and Gabriela Irazoqui.
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The aim of the journal article 'Covalent immobilization of tobacco-etch-virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins' is to develop a method for efficiently removing the histidine tag from recombinant proteins. The authors describe the covalent immobilization of tobacco-etch-virus NIa protease to a solid support, and demonstrate that this immobilized protease can effectively cleave histidine tags from recombinant proteins. They validate the effectiveness of this tool through a series of experiments, and suggest that it could be a useful tool for the production of purified recombinant proteins for downstream applications.