Write a paragraph (no more than 200 words, excluding references) describing the automated sequencing technique. Use the sources mentioned below and describe in your own words how automated sequencing is used by molecular biologists (for what purpose?) and the basic steps involved in performing this technique (you should also include the purpose of the key steps). For full marks, while you are describing the basic steps, you should also include the purpose of key components of a sequencing reaction—e.g. for what purpose is the primer and other components included in the reaction?
This is what I have so far...I was wondering whether someone can tell me if I'm on the right track or if I've made a mistake:
The Automated Sequencing technique is used to determine the amino acid sequence of proteins (1). In a test tube template DNA, primers (definition), deoxyribonculeotide triphosphate (dNTPs) and dideoxyribonculeotide triphosphate (ddNTPs, derivative of DNTPs but lack the 3’ hydroxyl group (2) are added. Then the primer is annealed and this allows the polymerase to elongate the nucleic acid chain in varying lengths until the last C nucleotide, upon which ddNTP is incorporated which prevents any further elongation of the chain (2). Each tube is run on gel.
I don't have the steps for the automated ones I'm just using the ones from the manual one...
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