The sequence of nitrogen bases in DNA is crucial because it is responsible for coding for specific traits. The order of these bases (adenine, thymine, cytosine, and guanine) determines the information encoded in genes, which ultimately dictates the synthesis of proteins. Proteins are essential for a wide range of functions in the body, including structural roles, catalyzing biochemical reactions as enzymes, and regulating biological pathways.
While the other options mentioned (coding for phosphates, determining if transcription will occur, and holding hydrogen bases together) relate to aspects of molecular biology, they do not capture the primary significance of the sequence of nitrogen bases itself.