God punished Adam and Eve primarily for disobeying His instructions regarding the Tree of Knowledge of Good and Evil. According to the biblical narrative in Genesis, God commanded them not to eat the fruit from this tree, but they chose to disobey and ate it anyway, influenced by the serpent. This act of disobedience is often viewed as a turning point that led to their expulsion from the Garden of Eden and introduced sin into the world.
The other options you mentioned are not the reasons cited in the scripture for their punishment. Their actions leading to Cain and Abel's story occur later in the narrative and are not directly related to their original disobedience in the garden.