The dark reactions, also known as the light-independent reactions or the Calvin cycle, are a part of photosynthesis that occurs in the stroma of chloroplasts. One key step in the Calvin cycle is the fixation of carbon dioxide (CO₂). During this process, CO₂ is incorporated into a five-carbon sugar called ribulose bisphosphate (RuBP) by the enzyme ribulose bisphosphate carboxylase/oxygenase (RuBisCO). This step leads to the production of three-carbon compounds (3-phosphoglycerate, or 3-PGA) that are eventually converted into glucose and other carbohydrates.
In summary, one part of the dark reactions is the carbon fixation step involving RuBisCO.
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