The true statement of eukaryotes but not of prokaryotes is:
They must contain a nucleus.
Eukaryotic cells have a distinct nucleus that houses their genetic material, while prokaryotic cells do not have a nucleus; instead, their genetic material is located in the cytoplasm. The other statements are not exclusively true for eukaryotes, as some eukaryotes can be unicellular, and they do contain membrane-bound organelles, which prokaryotes do not. Additionally, eukaryotes can be unicellular or multicellular.