The true statement is:
The human body uses 20 amino acids to form all types of proteins.
Here's a brief explanation of why the other statements are false:
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tRNA is attached to specific proteins in the cell’s cytoplasm.: tRNA (transfer RNA) is not attached to proteins; rather, it carries specific amino acids to the ribosome during protein synthesis.
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Amino acids do not need to be assembled in a particular order.: This is false because the sequence of amino acids in a protein (polypeptide chain) is critical for its structure and function.
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Amino acids form a polypeptide chain via carbon-carbon bonds.: Amino acids form peptide bonds (which involve a carbon and nitrogen atom) between the carboxyl group of one amino acid and the amino group of another, rather than directly via carbon-carbon bonds.