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Which of the following was/were in the Primer Mix of our PCR activity? Select all that apply, and choosing incorrect answers will cause a penalty.

Question 21 options:

template DNA from an insect not infected with Wolbachia

primers for the insect gene

dNTPS

DNA polymerase

primers for the Wolbachia gene

template DNA from an insect infected with Wolbachia

1 answer

In a typical PCR (Polymerase Chain Reaction) setup, the primer mix includes the following components:

  1. Primers for the target genes: These are short sequences of nucleotides that bind to the template DNA and initiate the synthesis of new DNA strands.
  2. dNTPs (deoxynucleotide triphosphates): These are the building blocks of DNA, consisting of adenine (A), thymine (T), cytosine (C), and guanine (G).
  3. DNA polymerase: This enzyme synthesizes new DNA strands by adding dNTPs to the growing chain.

Based on the provided options, the following would typically be included in the primer mix for a PCR activity:

  • Primers for the insect gene
  • dNTPs
  • DNA polymerase
  • Primers for the Wolbachia gene

The template DNA (both from insects infected and not infected with Wolbachia) would be included in the PCR reaction, but they do not belong to the primer mix itself. Therefore, you should not select "template DNA from an insect not infected with Wolbachia" and "template DNA from an insect infected with Wolbachia" as they are not part of the primer mix.

So the correct selections are:

  • Primers for the insect gene
  • dNTPs
  • DNA polymerase
  • Primers for the Wolbachia gene