All of the provided options can indicate that a chemical change has occurred, but some are more direct indicators than others.
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Monitor for the release of a gas - This is a common indicator of a chemical change, especially if gas was not present before the substances came into contact.
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Conduct a test to determine the chemical makeup of the molecules - This is a more complex method and would require specialized equipment; it doesn't provide immediate evidence of a change.
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Observe a color change - A color change can be a clear indicator of a chemical change, as it often signifies the formation of new substances.
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Test for the release of heat - This can also indicate a chemical change, particularly in exothermic reactions.
Among these options, the most reliable and immediate signs of a chemical change would be monitoring for the release of a gas and observing a color change. However, since you're looking for one response, observe a color change is often a straightforward and reliable observation of a chemical change.