The excerpt that contains a specific grievance of the American colonists included in the Declaration of Independence is:
“He has dissolved Representative Houses repeatedly, for opposing with manly firmness his invasions on the rights of the people.”
This line addresses the colonists' frustration with King George III's actions, specifically his dissolution of legislative bodies that opposed his rule, reflecting the broader grievances that led to the desire for independence. The other excerpts, while significant in the context of the Declaration, do not detail specific grievances.