The event that had the most impact on the growing tensions between the colonists and Great Britain was the French and Indian War.
This war (1754-1763) led to significant British debt, prompting Britain to impose taxes on the colonies to help pay for the war and its subsequent military presence in North America. The introduction of various taxes and laws, such as the Stamp Act and the Townshend Acts, sparked widespread resentment and resistance among the colonists, ultimately contributing to the tensions that led to the American Revolutionary War.
While the other events listed also contributed to colonial dissatisfaction, the aftermath of the French and Indian War was pivotal in escalating tensions significantly.