A substitution mutation occurs when one base in the DNA sequence is replaced by another.
Looking at the given sequences:
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C G T C T A C T G C A A T
G C A G A T G A C G T T A
→ This sequence shows multiple substitutions (G instead of T in the first sequence and A instead of C in the second).
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C G T T A C T G C A A T
G C A A T G A C G T T A
→ This is the original sequence, so it does not show any mutations.
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C G T A C T G C A A T
G C A T G A C G T T A
→ This sequence shows substitutions (A instead of T in the first sequence and T instead of C in the second).
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C G T G A C T G C A A T
G C A C T G A C G T T A
→ This sequence shows substitutions (G instead of T in the first sequence and C instead of A in the second).
Based on the options provided, the sequence that shows a substitution mutation is:
C G T A C T G C A A T
G C A T G A C G T T A
This indicates a substitution mutation occurred where T was substituted for A in the first strand and C was substituted for A in the second strand.