The correct choice is:
Prokaryotes tend to only have one chromosome while eukaryotes have many.
Here’s a brief explanation of the other choices:
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Prokaryotic chromosomes are typically much larger than eukaryotic ones. This is incorrect; prokaryotic chromosomes are generally smaller than eukaryotic chromosomes.
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Prokaryotic chromosomes are wrapped into structures called nucleosomes. This is incorrect; nucleosomes are part of the chromatin structure in eukaryotic cells, not prokaryotic cells.
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Prokaryotic chromosomes are linear while eukaryotic chromosomes are circular. This is also incorrect; prokaryotic chromosomes are typically circular, while eukaryotic chromosomes are linear.
Thus, the first statement is the only correct comparison.