Which lists the correct order of steps involved in the formation of an enzyme-substrate complex?

Answer 1

The formation of an enzyme-substrate complex involves several key steps. Here is the correct order of those steps:

1. Substrate Binding: The substrate molecule approaches and binds to the enzyme's active site. This can occur through various interactions such as hydrogen bonds, ionic bonds, or hydrophobic interactions.

2. Induced Fit: Once the substrate is bound to the active site, the enzyme undergoes a conformational change. This change enhances the fit between the enzyme and the substrate, stabilizing the enzyme-substrate complex.

3. Formation of the Enzyme-Substrate Complex: The combination of the enzyme and substrate forms the enzyme-substrate complex (ES) in which the substrate is positioned in a way that facilitates the chemical reaction.

4. Catalysis: The enzyme catalyzes the conversion of the substrate into the product(s) through various mechanisms, which may involve the lowering of the activation energy required for the reaction.

5. Release of Product: After the reaction occurs, the product(s) are released from the active site, which allows the enzyme to revert to its original shape and be free to bind with another substrate molecule.

6. Recycling of the Enzyme: The enzyme is now available to catalyze additional reactions with new substrate molecules.

This sequence highlights the dynamic interaction between the enzyme and substrate, leading to the transformation of reactants into products.