The correct response is:
They are part of the lined-up chromosomes in mitosis.
Sister chromatids are the identical copies of a single chromosome that are connected by a centromere and are present during mitosis when chromosomes line up at the metaphase plate. They do not form during metaphase; rather, they are already formed during the S phase of the cell cycle when DNA replication occurs. Sister chromatids separate during anaphase of mitosis to ensure that each daughter cell receives an identical set of chromosomes.