The context that describes a difference of rational numbers is:
"A football player loses a yard on a play."
This context involves comparing two quantities related to the yardage of the player, where the difference can be represented by subtracting the current yardage from the desired yardage (e.g., starting at 0 yards and losing 1 yard results in a difference of -1).
While the other options involve situations where scores or yardage change, they are not focused specifically on the concept of calculating a difference in a negative direction.