when designing a protocol to produce auxotrophs for biochemical pathways, how long should you irradiate yeast in this system to maximize the number of auxotrophic mutants? Explain your reasoning.

Answer 1

To maximize the number of auxotrophic mutant should not be more than 20 min, because if the cells exposed more than 20 min according to data I have, the number decrease start from 40 min and more.