The fundamental question addressed in each of the listed congressional acts corresponds to the following:
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Should the U.S. expand westward? - This is primarily associated with discussions around acts like the Homestead Act and issues related to Manifest Destiny.
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Should states be allowed to nullify federal laws? - This relates to the Nullification Crisis and discussions surrounding the Kentucky and Virginia Resolutions.
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Should slave labor be allowed in new states? - This is addressed in acts like the Missouri Compromise and the Kansas-Nebraska Act, which attempted to resolve the issue of slavery's expansion into new territories.
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Should states be able to secede from the Union? - This question became prominent during the Civil War, with no specific act directly addressing it, but the broader context involves the debates surrounding states’ rights versus federal authority.
Overall, the act most directly linked to the question of slave labor in new states would clearly be centered around the issue of slavery's expansion, which was a significant and contentious topic in the 19th century leading up to the Civil War.