For Thin Layer Chromatography (TLC) of dibenzalacetone, a common solvent system to use is a mixture of hexane and ethyl acetate. A typical starting ratio might be 4:1 (hexane:ethyl acetate), but you may need to adjust this depending on the polarity of your sample and the stationary phase used on your TLC plate.
Here’s a simple procedure:
- Prepare your TLC plate with a suitable stationary phase, such as silica gel.
- Spot your sample of dibenzalacetone on the TLC plate.
- Develop the plate in a saturated chamber with your solvent mixture.
- After the run is complete, visualize the spots using UV light or by applying a suitable staining reagent.
Always perform a test run with a small amount of your sample and adjust your solvent system based on the Rf values you obtain.
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