What is the procedure and discussion for Microscopic observation of Hibiscus Rosa sinensis flower and pteridophytes sorus?

Answer 1

Preparation of Hibiscus Rosa sinensis flower:

1. Collect a fresh Hibiscus Rosa sinensis flower.
2. Cut a thin cross-section of the flower using a sharp scalpel or razor blade.
3. Place the section on a glass slide and add a drop of water to prevent drying out.
4. Cover the section with a cover slip and gently press down to flatten the sample.

Microscopic observation of Hibiscus Rosa sinensis flower:

1. Place the prepared slide on the stage of a compound light microscope.
2. Start with the lowest magnification objective lens and focus on the section to get a general overview.
3. Gradually increase the magnification to observe the details of the flower, such as the different parts like petals, sepals, stamens, and pistil.
4. Take note of the arrangement of cells, their shape, size, and any other structural characteristics that are visible under the microscope.

Preparation of pteridophytes sorus:

1. Collect a mature frond of a pteridophyte plant that has visible sorus (clusters of sporangia).
2. Cut a thin cross-section of the sorus using a sharp scalpel or razor blade.
3. Place the section on a glass slide and add a drop of water to prevent drying out.
4. Cover the section with a cover slip and gently press down to flatten the sample.

Microscopic observation of pteridophytes sorus:

1. Place the prepared slide on the stage of a compound light microscope.
2. Start with the lowest magnification objective lens and focus on the section to get a general overview of the sorus.
3. Gradually increase the magnification to observe the sporangia inside the sorus.
4. Take note of the arrangement of sporangia, their structure, and any other characteristics that are visible under the microscope.

Discussion:

1. The Hibiscus Rosa sinensis flower is a typical dicot flower with all the essential parts such as petals, sepals, stamens, and pistil. Observing the flower under the microscope allows for a closer examination of the cellular structure of these parts.
2. Pteridophytes are a group of vascular plants that reproduce through spores produced in sporangia. Observing the sorus of a pteridophyte frond under the microscope provides insights into the reproductive structures of these plants.
3. Microscopic observation of plant structures like flowers and sorus helps in understanding their morphology, anatomy, and reproductive mechanisms, which are essential for plant classification and study.