What is the concentration of DNA in a solution if the corrected absorbance is 2.49?

You may copy/paste the following to indicate micro- μ. Report your answer to one decimal place.



Your Answer:
Question 14 options:
Answer ; units

1 answer

To calculate the concentration of DNA in a solution based on absorbance, you can use the following formula, which relates absorbance to concentration:

\[ \text{Concentration (μg/mL)} = \text{Absorbance} \times \text{Dilution Factor} \times \text{Conversion Factor} \]

For DNA, the typical conversion factor is 50 μg/mL per absorbance unit at 260 nm.

Given that the corrected absorbance is 2.49, and assuming the dilution factor is 1 (if the solution is not diluted), the calculation would be:

\[ \text{Concentration} = 2.49 \times 50 , \text{μg/mL} \]

\[ \text{Concentration} = 124.5 , \text{μg/mL} \]

Rounding to one decimal place, the concentration of DNA in the solution is:

Your Answer: 124.5 μg/mL