What is the concentration of DNA in a solution if its absorbance at 260 nm is 0.412? (Remember the appropriate constant to use: 50 ng of DNA/microliter of solution).

Answer 1

To calculate the concentration of DNA in a solution based on its absorbance at 260 nm, you can use the following formula:

\[ \text{Concentration (ng/µL)} = \text{Absorbance} \times \text{Constant} \]

Where the constant for DNA is 50 ng/µL (this is the amount related to 1 absorbance unit at 260 nm).

Given the absorbance (A) is 0.412, we can plug this value into the formula:

\[ \text{Concentration (ng/µL)} = 0.412 \times 50 , \text{ng/µL} \]

Calculating this:

\[ \text{Concentration (ng/µL)} = 20.6 , \text{ng/µL} \]

Therefore, the concentration of DNA in the solution is 20.6 ng/µL.