what is orcinol colour reaction

1 answer

The orcinol color reaction is a test used to detect the presence of various compounds, including sugars and phenolic compounds. It is commonly used in biochemistry and chemistry laboratories.

In this reaction, orcinol (a chemical compound) reacts with substances containing specific functional groups to produce a blue-colored product. The reaction is primarily used to detect the presence of carbohydrates, particularly reducing sugars such as glucose and fructose.

When a reducing sugar is present, it reacts with orcinol in the presence of an acidic solution (such as sulfuric acid) and heat. This reaction leads to the formation of a blue-colored complex. The intensity of the blue color is proportional to the amount of reducing sugar present.

Apart from detecting reducing sugars, the orcinol reaction can also be used to detect the presence of phenolic compounds, such as catechol and tyrosine. These compounds react with orcinol to form a green or blue-green color.

Overall, the orcinol color reaction is a useful and common method for identifying and quantifying reducing sugars and phenolic compounds in various samples.