What influence would a SNP have on the results of an RFLP analysis?

Answer 1

A SNP (Single Nucleotide Polymorphism) could potentially have a significant influence on the results of an RFLP (Restriction Fragment Length Polymorphism) analysis. This is because RFLP analysis relies on the presence or absence of specific restriction enzyme recognition sites within a DNA fragment, which in turn affects the lengths of the resulting fragments. Since SNPs represent single nucleotide differences between individuals, they can alter the location of restriction enzyme recognition sites and thereby affect the pattern of DNA fragments produced during the RFLP analysis. As a result, the presence of a SNP in a specific location can lead to a different RFLP pattern, potentially altering the interpretation of the results.