We cut a slice of the target gene from the gel which ran electrophoresis. And then purified the gene from the gel by using Gel-M column, WN buffer and WS buffer. After these steps, we ran another electrophoresis of the purified gene and there was no band showed on the picture. What might happened to make this situation happened?

Question

We cut a slice of the target gene from the gel which ran electrophoresis. And then purified the gene from the gel by using Gel-M column, WN buffer and WS buffer. After these steps, we ran another electrophoresis of the purified  gene and there was no band showed on the picture.  What might happened to make this situation happened?