To determine the concentration of substance Z based on the A600 measurement, we would typically use the Beer-Lambert Law, which is generally represented as:
\[ A = ε \cdot c \cdot l \]
Where:
- \( A \) is the absorbance (A600 in this case)
- \( ε \) is the molar absorptivity (a constant specific to the substance at the given wavelength)
- \( c \) is the concentration of the substance
- \( l \) is the path length of the cuvette (typically in cm)
Without specific values for \( ε \) and \( l \), we can't calculate the concentration directly. However, if you have a standard curve or a specific equation that relates A600 to concentration, please provide that, and I can help you compute the concentration of substance Z.
If you plug in the values into a known equation or if you've provided a context to find those values, please provide it for further assistance!