Based on your options and the context of gene expression, the most appropriate response is:
Positive transcription factor affinity has a greater influence because you cannot turn genes on without it.
While polymerase affinity is essential for the transcription of mRNA, positive transcription factors are crucial for initiating transcription by helping to recruit polymerase and ensuring that specific genes are turned on. Thus, the activity and affinity of transcription factors prominently influence the levels of protein expressed from genes.