The observation that most likely led to the reclassification of the myrtle warbler and the Audubon's warbler as one species is:
the myrtle warbler and the audubons warbler interbreed and produce fertile offspring.
This observation is crucial in biological classification, as the ability to interbreed and produce fertile offspring is a key criterion for defining a species. If two populations can successfully mate and their offspring are viable and fertile, it indicates that they are not separate species but rather variations within the same species.