Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 10-6 for the following aqueous reaction.
(HOCH2)3CNH2 + H2O (HOCH2)3CNH3+ + OH -
(HOCH2)3CNH2=TRIS (HOCH2)3CNH3+=TRISH +
What is the pH after 0.59 mL of 12 M HCl is added to a 200.0 mL portion of the buffer?
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