To determine v max and km of Beta-galactosidase for lactose, the same amount of enzyme (1 ug per tube) was incubated with a series of lactose concentrations under conditions where product concentrations remained negligible. At each lactose concentration, the initial reaction velocity was determined by assaying for the amount of lactose remaining at the end of the assay.

Question

Question: Calculate 1/v and 1/[s] for each entry. (Please note the equations used.)

Lactose concentration (m/M) (believe is [s])
1
2
4
8
16
32
Rate of lactose consumption (u mol/min) (believe is v)

10.0
16.7
25.0
33.3
40.0
44.4

(Please show me how to do aleast the first two. And the formulas used.)

Question: When graphing is 1/v on the y-axis and 1/[s] on the y axis?