Then, extraction of active compounds took place. After 4 weeks, the mixture containing dichloromethane and Tithonia diversifolia taken from the Zocki Zoi area in Goroka, EHP was placed on a heater or heating plate to evaporate the solvents and extract the antibacterial compounds contained in the crude aqueous extracts. This concentrated extract was further processed using a rotary evaporator.

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Following the extraction of active compounds, the mixture containing dichloromethane and Tithonia diversifolia, sourced from the Zocki Zoi area in Goroka, EHP, was subjected to a 4-week extraction period. Afterward, the mixture was placed on a heating plate to evaporate the solvents and concentrate the antibacterial compounds from the crude aqueous extracts. This concentrated extract was subsequently further processed using a rotary evaporator.