Based on the information provided, the statement that is true is:
Many different Native American nations were forcibly removed after the Indian Removal Act passed.
This is because the Indian Removal Act led to the removal of various tribes, including but not limited to the Cherokee, Choctaw, Chickasaw, Seminole, and Muscogee. The other statements are incorrect; the Cherokee Nation was not the first to be forcibly removed under the act, and there were other tribes that were also affected beyond just those listed.