The correct answer is D. all of these.
Each of the listed options represents a potential limitation that the scientist may face when designing a solution to increase the gopher tortoise population.
A. The environmental impact of the design solution on other species could affect the ecosystem balance.
B. The cost and overall economic impact of the design solution can limit its feasibility and implementation.
C. The willingness of local inhabitants to stop using rattlesnake removal methods that harm tortoises will be crucial for the success of any conservation efforts.
Therefore, all these factors can be limitations to the scientist's design.