The components of a 11.0 mg sample are sufficiently separated by a chromatographic column that is 1.4 cm in diameter and 50 cm long with a flow rate of 0.57 mL/min. In order to separate 271 mg of the sample with similar separation of the components. What size column, diameter and length, should be used? And what volume flow rate should be used?

Column Diameter= ___cm
Column Length= ___cm
Flow Rate= ___mL/min

1 answer

Column Diameter= 5.6 cm
Column Length= 280 cm
Flow Rate= 2.4 mL/min