The article describes a PCR test designed to detect fungi that cause keratitis. What type of patient samples did the researchers extract DNA from for the PCR analysis?

Question

Question 26 options:

corneal scrapings

skin swabs

fecal samples

saliva samples

Question 27 (2 points)

In this study, what fungal gene was the PCR specifically detecting?

Question 27 options:

16S rRNA gene

18S rRNA gene

CO1 gene

helicase gene

Question 28 (2 points)

What is TRUE regarding the conditions used in this PCR test?

Question 28 options:

The annealing temperature used was 60°C

The denaturation step was 60 seconds long

The PCR program contained 30 amplification cycles

They did not run a positive control

Question 29 (2 points)

What was the approximate size of the PCR product in base pairs (bp)?

Question 29 options:

70,000

1000

400

30

Answer 1

Based on the information you provided regarding the PCR test designed to detect fungi causing keratitis, here are the answers to your questions:

Question 26: What type of patient samples did the researchers extract DNA from for the PCR analysis?

• Answer: corneal scrapings

Question 27: In this study, what fungal gene was the PCR specifically detecting?

• Answer: 18S rRNA gene

Question 28: What is TRUE regarding the conditions used in this PCR test?

• Answer: The PCR program contained 30 amplification cycles

Question 29: What was the approximate size of the PCR product in base pairs (bp)?

• Answer: 400

These answers should provide the necessary information regarding the PCR test described in the article. If you have further questions or need additional information, feel free to ask!