summasrise this for BCL11A only and the corrected C46 cells expressed KLF1 and BCL11A transcripts at lower levels compared to those of the peripheral blood-derived erythroblasts. Similarly, analysis of beta and alpha hemoglobin protein expressions showed that both the Eβ-iPSC2 cells and the corrected C46 cells expressed the proteins at similar levels; however, the beta hemoglobin levels were much lower than those of the peripheral blood-derived erythroblasts. A previous study demonstrated that the transcription factors KLF1 and BCL11A are required for the induction of beta hemoglobin levels [38]; therefore, low levels of KLF1 and BCL11A transcripts in the corrected C46 cell-derived erythroid cells could result in lower beta hemoglobin expression.
1 answer
The corrected C46 cells exhibited lower levels of KLF1 and BCL11A transcripts compared to peripheral blood-derived erythroblasts, leading to reduced beta hemoglobin expression. This suggests that KLF1 and BCL11A are crucial transcription factors for inducing beta hemoglobin levels in erythroid cells.