Students conducting research observe the rate of an enzyme-catalyzed reaction under various conditions with a fixed amount of enzyme in each sample. When will increasing the substrate concentration likely result in the greatest increase in the reaction rate?

when a noncompetitive inhibitor is present in the reaction
when a competitive inhibitor is present in the reaction
when a cofactor is required in the reaction
when an allosteric inhibitor is present in the reaction

1 answer

To determine when increasing the substrate concentration will likely result in the greatest increase in the reaction rate, we need to consider how each condition affects the rate of an enzyme-catalyzed reaction.

  1. When a noncompetitive inhibitor is present in the reaction: Noncompetitive inhibitors bind to an enzyme regardless of whether the substrate is present. This means that even with increased substrate concentration, the maximum reaction rate can't be greatly affected because some enzyme molecules are still inhibited.

  2. When a competitive inhibitor is present in the reaction: Competitive inhibitors compete with the substrate for the active site. Increasing substrate concentration can help overcome the effect of the competitive inhibitor, leading to a greater increase in the reaction rate as more substrate molecules are available to outcompete the inhibitor.

  3. When a cofactor is required in the reaction: If the reaction requires a cofactor, the presence of this cofactor is essential for the enzyme to function, but substrate concentration can still significantly affect the reaction rate, particularly when the enzyme is active.

  4. When an allosteric inhibitor is present in the reaction: Allosteric inhibitors bind to the enzyme at a site other than the active site, leading to a change in the enzyme's shape and function. Increasing substrate concentration does not have as strong an effect in this case, as the inhibition state of the enzyme prevents effective catalysis.

Given this information, the greatest increase in the reaction rate with increasing substrate concentration is likely to occur when a competitive inhibitor is present. This is because increasing substrate concentration can help alleviate the inhibitory effects of the competitive inhibitor, leading to a higher reaction rate as more enzyme active sites are occupied by the substrate.