Staphylococcal nuclease (Staph Nuc) is a heat-stable, potent secretory protein that cleaves dsDNA in vitro. You have a cloned the Staph Nuc gene on pBR322 plasmid and transferred into E. coli. You have named the chimeric plasmid pBR322 with the cloned Staph nuc gene as pNuc. In an experiment, it was necessary that you purify pNuc from an E. coli culture, but was unsuccessful. The cause for being unable to purify the pNuc plasmid was that the Staphylococcus nuclease enzyme hydrolyzing the plasmid along with the chromosomal DNA immediately after cell lysis and released in the salt buffer during purification process. How would solve this issue and protect the pNuc plasmid DNA so that you can proceed with the downstream molecular analyses of the gene?
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