Slide 1: Create a slide that contains a table that presents the results from your DNA extraction from Exercise 5.

Question

Make a table on the slide using the tools within Powerpoint or Google.
Do NOT insert a screenshot of the respective table from your lab manual.
Create an appropriate, descriptive title for the slide.
Hint: “Results” doesn’t tell you much! Would you want a newspaper headline to read “Election Happened,” or do you want to know who won the election?

Include ONLY the following information on the slide:
Insect Specimen Identification (example: MEH12F18-1)
Insect Specimen Order Name (My specimen order is Diptera)
Extracted DNA Concentration (with correct units) A260=0.7; A280=0.42
Extracted DNA A260/A280 ratio (find the ratio given 0.7 and 0.42 from above)
Don’t report as a fraction: divide to get a number. For example, if your A260 is 2.5 and your A280 is 1.25, then your A260/A280 =2.0.
Extracted DNA Quality
Hint: Is your sample clean or not? How do you know? What else could be in abundance in your sample besides DNA?

Answer 1

Slide Title: DNA Extraction Results from Diptera Specimen MEH12F18-1

| Insect Specimen Identification | Insect Specimen Order | Extracted DNA Concentration (A260/A280) | Extracted DNA A260/A280 Ratio | Extracted DNA Quality | |--------------------------------|-----------------------|-----------------------------------------|-----------------------------|----------------------------| | MEH12F18-1 | Diptera | A260=0.7; A280=0.42 | 1.67 | Moderate Quality (Impurities Present) |

Notes for Slide:

• Extracted DNA Concentration: Indicate the absorbance values at 260 nm and 280 nm, which are essential for determining DNA concentration.
• Extracted DNA A260/A280 Ratio: Calculate the ratio based on the provided values (0.7 / 0.42 = 1.67).
• Extracted DNA Quality: Mention that the A260/A280 ratio indicates the presence of impurities; a ratio of around 1.8 is ideal for pure DNA, so a ratio of 1.67 suggests moderate quality with some impurities (possibly proteins or other contaminants).

Additional Formatting Suggestions:

• Use a clear font and color scheme that contrasts well for readability.
• Ensure that the table is centered on the slide for visual balance.
• Consider adding a brief note below the table to explain what the A260 and A280 readings mean for those less familiar with DNA extraction processes.