Inside the mitochondrion, several key biochemical processes occur that are essential for cellular energy production and metabolism. Here’s a summary of what happens inside a mitochondrion:
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Energy Production (ATP Synthesis):
- The primary function of mitochondria is to produce adenosine triphosphate (ATP), which serves as the energy currency of the cell.
- This process mainly occurs in the inner mitochondrial membrane through a series of reactions known as oxidative phosphorylation.
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Citric Acid Cycle (Krebs Cycle):
- In the mitochondrial matrix, pyruvate (produced from glucose during glycolysis) is further broken down in the citric acid cycle.
- This cycle produces electron carriers (NADH and FADH2) and small amounts of ATP.
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Electron Transport Chain:
- The NADH and FADH2 generated from the citric acid cycle are used in the electron transport chain, located in the inner mitochondrial membrane.
- Electrons are transferred through a series of protein complexes, releasing energy that pumps protons (H+ ions) into the intermembrane space, creating a proton gradient.
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Chemiosmosis and ATP Synthase:
- The protons flow back into the mitochondrial matrix through ATP synthase, a protein complex that synthesizes ATP from ADP and inorganic phosphate.
- This process of using the proton gradient to generate ATP is known as chemiosmosis.
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Role in Metabolism:
- Mitochondria are involved in the metabolism of fatty acids and amino acids, contributing to energy production and biosynthesis.
- They also play a role in the regulation of the metabolic pathways that manage the cell's energy balance.
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Apoptosis (Programmed Cell Death):
- Mitochondria are involved in apoptosis, releasing cytochrome c and other factors that activate the apoptotic pathways when the cell is damaged or under stress.
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Heat Production:
- In some specialized cells, such as brown adipose tissue, mitochondria are involved in thermogenesis (heat production) through a process called non-shivering thermogenesis.
Mitochondria are crucial for metabolism, energy production, and various cellular functions, making them vital organelles in nearly all eukaryotic cells.