I’m curious about how recombinant DNA technology can be leveraged to tackle genetic disorders that stem from mutations in single genes. Specifically, I’m wondering about the methodologies involved in identifying the mutation and how this technology can be applied to potentially correct or mitigate the effects of that mutation.
For instance, I would like to understand the processes behind techniques like CRISPR-Cas9 and how they can be used to target and edit specific genes. What are the potential challenges we might face in ensuring that the changes made do not have unintended consequences? Also, how do we ensure that the treatments developed are safe and effective before they can be widely implemented?
Any insights or examples of current research in this area would be greatly appreciated, as I am eager to grasp the practical applications of recombinant DNA technology in treating genetic disorders.