Enslaved people who were taken to the American colonies experienced severe hardships and trauma. The correct response among the options you've listed is:
They were separated from their families.
During the transatlantic slave trade, individuals were often taken from their families and communities, leading to profound and lasting psychological and emotional impacts. While enslaved individuals faced brutal conditions and often inadequate food and water during the Middle Passage, they were not asked about their preferences for work, nor were they typically allowed to work with friends to maintain morale.