Question 18 options:

Question

Now, let's analyze a substance different from DNA. You have a solution made from the hypothetical substance Z. The constant for substance Z at a wavelength of 600nm is 80 ng of substance Z/μL of solution. Write the equation that you should use to determine the concentration of substance Z if you are given an A600.

Hint: An equation "equates" two entities. If you do not include two things separated by an "=", then you have not written an equation -- you will have simply written a mathematical operation.

Answer 1

To determine the concentration of substance Z based on its absorbance at 600 nm (A600), we can use Beer-Lambert's law, which relates absorbance to concentration. The general form of this equation is:

\[ A = k \cdot C \]

Where:

• \( A \) is the absorbance (A600 in this case),
• \( k \) is the proportionality constant (which is the specific constant for substance Z at the given wavelength),
• \( C \) is the concentration of the substance.

Given that the constant for substance Z at 600 nm is 80 ng/μL, the equation can be written as:

\[ A600 = 80 , \text{ng/μL} \cdot C \]

This means:

\[ A600 = k \cdot C \text{ or } A600 = 80 \cdot C \]

To solve for the concentration \( C \), you can rearrange the equation:

\[ C = \frac{A600}{80 , \text{ng/μL}} \]

So the final equation to determine the concentration of substance Z based on the absorbance A600 is:

\[ A600 = 80 \cdot C \]